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TitleInvestigatory Project
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                            “On The Antipyretic, Anti-Inflammatory, Analgesic And Molluscicidal Properties Of Polyscias Fruticosa (L) Harms”
“A Pharmacognostic Report On The Leaf And Root Of Polyscias Fruticosa (L.) Harms”
	Polyscias Fruticosa-Medicinal Tonic
“A Pharmacognostic Report on the Leaf and Root of Polyscias Fruticosa (L.) Harms”
Document Text Contents
Page 1

San Miguel National High School

Scuala St., San Juan, San Miguel, Bulacan

Phytochemical Screening of Papua

Leaves (Polyscias fruticosa)

An Investigatory Project Presented
To The Department Of Science –
San Miguel National High School

In Partial Fulfillment Of The
Requirements In Technical Writing II

Submitted to:

Mrs. Marinella P. Garcia Sy

Submitted by:

Princess Fay S. Lopez

Mark Ranniel M. Estarez

IV- Science Class (B)

October 2012

Page 2

The importance of plants is known to us well. The plant kingdom is a treasure house of

potential drugs and in the recent years there has been an increasing awareness about the

importance of medicinal plants. Drugs from the plants are easily available, less expensive, safe,

and efficient and rarely have side effects.
Papua Plant Leaves were air dried and washed with distilled water. It was cut into

pieces and turned into smaller pieces using the domestic blender. The extract was obtained and

transferred to Beaker using cheese cloth.

The extract was then screened for the presence of nine phytochemicals which were

alkaloids, carbohydrates, glycosides, saponins, phenols, tannins, anthocyanin, protein and


The result revealed the abundance of flavonoids, saponins and proteins. There were

traced amounts of carbohydrates, alkaloids, phenols and anthocyanin. However, glycosides and

tannins were absent in the plant extract.

Based on the results of the phytochemical screening, properties and uses of Papua

Plant extract could be used in the industry such as foaming agents, wood and plastic production

as well as other chemical processes.


Page 25

B.1. Preparation of Pure Extract, Reagents and Solutions
Papua Plant Leaves were air dried and washed with distilled water. It was cut into pieces

and turned into smaller pieces using the domestic blender. The extract was obtained and

transferred to Beaker using cheese cloth.


Fig 6 Pure Papua Plant Extract

B.2. Preparation of Reagents, Solutions and Chemicals for Phytochemical Profiling

B.2.1 Prepare the Chemicals Needed

Fig. 7 Chemicals Needed

B.2.2 Mayer’s Reagent (Reagent for Alkaloid Test)
0.4 g of Mercuric Chloride (HgCl2) is weighed and dissolved in 15 mL of water (H2O). On

one hand, 1.5 g of Potassium Iodide (KI) is dissolved in 2.5 mL of water (H 2O). Sufficient

water is added to make the volume 25 mL A fraction of the reagent was obtained and placed

in a test tube.

Fig. 8 0.4 g Mercuric Chloride Fig. 9. Mercuric Chloride Aqueous Solution

Page 26

Fig. 10 1.5 g Potassium Iodide Fig. 11. Potassium Iodide Aqueous Solution

Fig. 12 Mayer’s Reagent
B.2.3 Molisch’s Reagent (Reagent for Carbohydrate Test)

A mass of 3.8 g of naphthol was obtained and dissolved in 25 mL of ethanol. A fraction of

the reagent was transferred into a test tube.

Fig. 13 3.8 g of Naphthol

Fig. 14 Naphthol to Water Fig. 15. Molisch’s Reagent
B.2.3 Acetic Acid and Ferric Chloride (Chemicals for Glycoside Test)

2.5 mL of acetic acid were transferred into a small beaker. In a separate container. 1.5 g

of ferric chloride was also prepared. The chemicals were set aside for further use.

Page 49

Flavonoids Antimicrobial


Complex with cell wall, binds to adhesins

Inhibits release of autocoids and prostaglandins,

Inhibits contractions caused by spasmogens,

Stimulates normalization of the deranged water

transport across the mucosal cells,

Inhibits GI release of acetylcholine
Polyphenols and





Binds to adhesins, enzyme inhibition, substrate

deprivation, complex with cell wall, membrane

disruption, metal ion complexation

Makes intestinal mucosa more resistant and

reduces secretion, stimulates normalization of

deranged water transport

across the mucosal cells and reduction of the

intestinal transit, blocks the binding of B subunit

of heat-labile enterotoxin to

GM1, resulting in the suppression of heat-labile

enterotoxin-induced diarrhea, astringent action

Increases supply of digestible proteins by

animals by forming protein complexes in rumen,

interferes with energy

generation by uncoupling oxidative

phosphorylation, causes a decrease in G.I.

Coumarins Antiviral Interaction with eucaryotic DNA
Terpenoids and

essential oils



Membrane disruption

Inhibits release of autocoids and prostaglandins

Page 50

Alkaloids Antimicrobial



Intercalates into cell wall and DNA of parasites

Inhibits release of autocoids and prostaglandins

Possess anti-oxidating effects, thus reduces

nitrate generation which is useful for protein

synthesis, suppresses transfer of

sucrose from stomach to small intestine,

diminishing the support of glucose to the

helminthes, acts on CNS causing

Lectins and


Antiviral Blocks viral fusion or adsorption, forms disulfide

Glycosides Antidiarrhoeal Inhibits release of autocoids and prostaglandins
Saponins Antidiarrhoeal



Inhibits histamine release in vitro

Possesses membrane permeabilizing properties

Leads to vacuolization and disintegration of

Steroids Antidiarrhoeal Enhance intestinal absorption of Na+and water

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